Objective: The aim of this study is to evaluate the normal and abnormal pericallosal artery (PCA) by three-dimensional (3D) ultrasonography and Doppler technologies. Additionally, it describes a systematic method on how to acquire and manipulate volumes for the precise demonstration of the PCA. Methods: With the use of the glass body mode (grayscale ultrasound and color or power Doppler), 3D ultrasound permits precise imaging of the fetal PCA, an important anatomic landmark of the corpus callosum. This rendering mode enables us to demonstrate sonographically the origin, course, and branching patterns of the PCA with the callosomarginal artery (CMA) as well as other fetal cerebral blood vessels and venous sinuses. Results: A step-by-step systematic approach to identifying the cerebral vasculature from ultrasonographic volume data sets was developed. The volumes were rotated into a standard anatomic orientation in the multiplanar display, and then, by systematic navigation, the PCA with its main branches was demonstrated. Conclusion: The use of 3D power Doppler sonoangiography enables the precise demonstration of the normal variants of the fetal brain vessels and provides knowledge about anatomical variations and the diversity of human brain anatomy. The knowledge of normal variants helps to detect pathological forms of brain vascularity.